Eastern Kentucky
Cooperators at the University of Kentucky have been planting chestnut in Pike County in eastern
KY since 2005 on tail-dumped spoil.  In that study, experimental plots were established on grey
sandstone, brown, weathered sandstone and run-of-the mine spoil.  Better growth is probably
 occurring on the brown sandstone and brown sandstone portions of mixed spoil than on grey
sandstone, but the difference is not yet statistically significant.  Most seedlings are chlorotic. 
The cooperators also are comparing direct seedling to planting of seedlings, and currently feel
that direct seeding is at least as good as planting seedlings.
 
Ohio
Cooperators at universities in Ohio have been planting chestnut on reclaimed mined lands since
2005.  They are evaluating direct seeding versus seedlings, mycorhizzal treatments and also are
comparing growth of backcross trees with varying fractions of American and Chinese
chestnut.  Independently, foresters with the Ohio Department of Natural Resources have
been planting pure American chestnut seedlings on reclaimed mined land since 2003.  The
seedlings had been inoculated or not inoculated with Pisolithus tinctorius, an
ectomycorrhizal fungus.  The seedlings had been established and inoculated by TACF
cooperators with the U.S. Forest Service in Delaware, OH. In some plantings but not others,
mycorrhizal inoculation increased growth and seedling height.