| The American Chestnut
FoundationÕs Breeding Program |
| Requirements for breeding |
| Materials |
| Materials Sources of blight resistance |
| Slide 5 |
| Slide 6 |
| 2006 Harvest in Meadowview |
| Slide 8 |
| Frequency Distribution of Percent American-Chestnut RAPD Alleles at First Backcross |
| Slide 10 |
| Expected distribution of canker phenotypes and genotypes in Chinese x American F2 progeny, based on observation and interpolation. |
| Backcrossing Need more than one American parent |
| Backcrossing Need more than one American chestnut background |
| Intercrossing of Third
Backcrosses How do we do it? |
| Slide 15 |
| Mean RAPD Score in Individual Trees by Linkage Group |
| Frequency Distribution of Number of Non-Recombinant Linkage Groups in Chestnut B1 |
| Statistics for Markers on Linkage Groups in Chestnut B1 |
| Intercrossing of Third
Backcrosses What mating design do we use? |
| Intercrossing of Third
Backcrosses Possible Mating Designs: Complete Diallel |
| Intercrossing of Third
Backcrosses Complete Diallel, No Selfing |
| Intercrossing of Third
Backcrosses Half Diallel, No Selfing |
| Intercrossing of Third
Backcrosses Disconnected Partial Half Diallels, No Selfing |
| Intercrossing of Third
Backcrosses Disconnected Partial Half Diallels, No Selfing Two Parents per Diallel |
| Intercrossing of Third
Backcrosses Connected Partial Half Diallels, No Selfing Two Parents = Circular Mating Design |
| Slide 26 |
| Intercrossing of Third
Backcross F2s Half Diallel, No Selfing, Two Parents per Diallel |
| Slide 28 |
| Intercrossing of Third
Backcross F3s Half Diallel, No Selfing, Two Parents per Diallel: Inbreeding Coefficients |
| Slide 30 |
| Slide 31 |
| Slide 32 |
| Slide 33 |
| Slide 34 |
| Conclusions |
| Cultivation Method |
| Slide 37 |
| Slide 38 |
| Slide 39 |
| Slide 40 |
| Slide 41 |
| Number of Trees at Meadowview in 2006 |
| Number of B3s by State in 2006 |
| Resistance |
| Slide 45 |
| Slide 46 |
| Slide 47 |
| Slide 48 |
| Resistance |
| Demonstration of statistically significant differences in canker length on American chestnut for virulent isolates of Cryphonectria parasitica |
| Slide 51 |
| Resistance |
| Resistance |
| Slide 54 |
| Blight-resistant Chinese to
American B2-F2, 5 years old, 2 years after inoculation with Cryphonectria parasitica. |
| Slide 56 |
| Blight-Resistant Clapper B3-F2 |
| Chestnut Blight Resistance Rating Scheme |
| Slide 59 |
| Slide 60 |
| Slide 61 |
| "Blight-resistant ÔClapperÕ x Ô..." |