Conclusions
¥Two progeny per B3 family gives lower inbreeding than one
¥At least nine to ten B3-F2 progeny are needed per B3 family to capture most alleles
¥If each of those nine to ten sets of B3-F2 progeny is segregated in a separate orchard, inbreeding at F4 is reduced
¥Inbreeding is not strongly affected by available Òmating designs,Ó in part because we use open pollination
¥The American Chestnut FoundationÕs breeding program will yield adequate effective population sizes (although the projections have a number of assumptions that will not hold true, decreasing the projected Ne)