Backcrossing
More than one American chestnut line also is needed, also to avoid inbreeding, when B3s are intercrossed with each other.
¥C  x A13
¥ø
¥F14 x A14
¥ø
¥B14 x A15
¥ø
¥B24 x A16
¥ø
¥B34
¥C  x A9
¥ø
¥F13 x A10
¥ø
¥B13 x A11
¥ø
¥B23 x A12
¥ø
¥B33   x
¥C  x A5
¥ø
¥F12 x A6
¥ø
¥B12 x A7
¥ø
¥B22 x A8
¥ø
¥B32
¥C  x A1
¥ø
¥F11 x A2
¥ø
¥B11 x A3
¥ø
¥B21 x A4
¥ø
¥B31   x
ø
B3-F212
ø
B3-F234